PhD in Biological Sciences and Applied Biotechnologies

Contacts

Phd thesis

Title: "Effect based monitoring tools: biomarkers for the assessment of emerging environmental risk factors for human health"

Tutor: Prof. Tiziana Schilirò

Main activities: 

- solid phase extraction of wastewater samples

- assessment of the estrogenic activity induced by environmental and biological samples (assays: E-screen assay and gene reporter assay, cells: MCF-7 and MELN)

- organic extraction of Particulate Matter samples 

- assessment of the cytotoxicity induced by Particulate Matter extracts (assays: WST-1 assay and LDH assay, cells: BEAS-2B)

- assessment of the genotoxicity induced by Particulate Matter extracts (assay: Comet assay, cells: BEAS-2B)

Research activities

Publications:

Marangon D., Traversi D., D'Agostino A.M., Gea M., Fontana M., Schilirò T. (2021). The North-western Italy air quality monitoring network: improving experience of PM2.5 assessment with mutagenicity assay. Environmental Research, 195: 110699. doi: 1016/j.envres.2020.110699

Zhang C., Schilirò T., Gea M., Bianchi S., Spinello A., Magistrato A., Gilardi G., Di Nardo G. (2020). Molecular basis for endocrine disruption by pesticides targeting aromatase and estrogen receptor, International Journal of Environmental Research and Public Health, 17: 5664. doi: 10.3390/ijerph17165664.

Gea M., Toso A., Schilirò T. (2020). Estrogenic activity of biological samples as a biomarker, Science of the Total Environment, 740: 140050. doi: 10.1016/j.scitotenv.2020.140050.

Spina F., Gea M., Bicchi C., Cordero C., Schilirò T., Varese G.C. (2020). Ecofriendly laccases treatment to challenge micropollutants issue in municipal wastewaters, Environmental Pollution, 257: 113579.doi: 10.1016/j.envpol.2019.113579.

Gea M., Bonetta Sa., Iannarelli L., Giovannozzi A.M., Maurino V., Bonetta Si., Hodoroaba V.D., Armato C., Rossi A. M., Schilirò T. (2019). Shape-engineered titanium dioxide nanoparticles (TiO₂-NPs): cytotoxicity and genotoxicity in bronchial epithelial cells, Food and Chemical Toxicology, 127:89 – 100. doi: 10.1016/j.fct.2019.02.043.

Gea M., Schilirò T., Iacomussi P., Degan R., Bonetta S., Gilli G. (2018). Cytotoxicity and genotoxicity of light emitted by incandescent, halogen and led bulbs on ARPE-19 and BEAS-2B cell lines, Journal of Toxicology and Environmental Health, Part A, 81 (19): 998 – 1014. doi: 10.1080/15287394.2018.1510350.

Villarini M., Levorato S., Salvatori T., Ceretti E., Bonetta S., Carducci A., Grassi T., Vannini S., Donato F., Bonetta Si., Verani M., de Donno A., Bonizzoni S., Bonetti A., Moretti M., Gelatti U., the MAPEC LIFE Study Group: Fatigoni C., Monarca S., Covolo L., Feretti D., Festa A., Viola G. C. V., Zani C., Zerbini I., Gilli G., Carraro E., Schilirò T., Pignata, C., Gea M., Romanazzi V., Donzelli G., Palomba G., Bagordo F., De Giorgi M., G.M., Idolo A., Panico A., Serio F., Verri T., Furia C., Colombi P., Codenotti R., Zagni, L. (2018), Buccal micronucleus cytome assay in primary school children: A descriptive analysis of the MAPEC_LIFE multicenter cohort study, International Journal of Hygiene and Environmental Health, 221 (6):883 – 892. doi: 10.1016/j.ijheh.2018.05.014

Bagordo F., De Donno A., Grassi T., Guido M., Devoti G., Ceretti E., Zani C., Feretti D., Villarini M., Moretti M., Salvatori T., Carducci A., Verani M., Casini B., Bonetta Sa., Carraro E., Schilirò T., Bonizzoni S., Bonetti A., Gelatti U., MAPEC_LIFE study group: Serio F., De Giorgi M., Idolo A., Verri T., Covolo L., Donato F., Festa A., Limina R. M., Zerbini I., Fatigoni C., Levorato S., Monarca S., Vannini S., Donzelli G., Bruni B., Palomba G., Bonetta Si., Gea M., Gilli G., Pignata C., Romanazzi V., Furia C., Codenotti R., Colombi P., Crottini S., Gaffurini L., Zagni L. (2017), Lifestyles and socio-cultural factors among children aged 6-8 years from five Italian towns: The MAPEC-LIFE study cohort, BMC Public Health, 17(1):233: 1-12. doi: 10.1186/s12889-017-4142-x.